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. 2014 Sep 11;9(9):e106904. doi: 10.1371/journal.pone.0106904

Figure 4. Effects of dobutamine (DOB) or vehicle (VEH) administration during hindlimb unloading (HU) or ambulatory cage activity (CC) on cancellous bone bone cell activity and marrow adipocyte proliferation.

Figure 4

(Top Row) Representative micrographs of osteoblasts (von Kossa and tetrachrome stain; ×400 magnification), (Middle Row) marrow adipocytes (x200 magnification), and (Bottom Row) fluorochrome labeling (calcein, 200×) on the surface of cancellous bone at the distal femur. Note the increased number of osteoblasts (black arrows, top row) and greater osteoid surface (below osteoblasts) in the HU+DOB group vs. HU+VEH. Also, note the increased number of marrow adipocytes (red arrows, middle row) in the HU+VEH group. The increased number of osteoblasts noted in the von Kossa and tetrachrome stained slides from HU+DOB samples correlated with extensive flurochrome labeling and large interlabel width (bottom row).