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. 2014 Sep 11;9(9):e106601. doi: 10.1371/journal.pone.0106601

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© 2014 Parsanejad et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) DJ-1 full length protein sequence. Peptide observed from DJ-1 after using PON2 as bait is highlighted. Mascot peptide score is 30.2. (B) HEK293 cells expressing GST-DJ-1 or GST as control were lysed and GST-DJ-1 was precipitated by glutathione sepharose beads and analyzed with Western blotting using PON2 antibody. (C) HEK293 cells were transfected with plasmid expressing Myc-PON2 (M-PON2). Cells were lysed and Myc-PON2 was precipitated with Myc antibody. Isolated complexes were analyzed with Western blotting using DJ-1 antibody. (D) DJ-1 was pulled down by DJ-1 antibody from cell lysate extracted from cultured cortical neurons. Immune complexes were analyzed with Western blotting using PON2 antibody.