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. 2014 Sep 11;9(9):e106992. doi: 10.1371/journal.pone.0106992

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© 2014 Shamsi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fat cells were collected at 4, 8, 12 and 16 weeks and immunofluorescence staining was used to analyze expression of CIDEC and PPAR-γ. Row A and C show expressions of PPAR-γ and CIDEC in mice fed on normal diet (ND) and 4, 8, 12 and 16 weeks of high fat diet (HFD) group. Row B and D represent effects of pioglitazone on the expressions of PPAR-γ and CIDEC in ND group, and 4, 8, 12 and 16 weeks of HFD group. Fluorescence microscope was used to observe the expressions and distribution of PPAR-γ and CIDEC.