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. 2014 Sep 11;9(9):e107036. doi: 10.1371/journal.pone.0107036

Figure 2. Arhgap28-V5 inhibits RhoA activation and stress fiber formation in SaOS-2 cells.

Figure 2

SaOS-2 cells were transfected with empty vector or Arhgap28-V5. A. The expression of Arhgap28-V5 was confirmed by western blotting using an antibody to V5. B. Effect of Arhgap28-V5 expression on the basal activity of RhoA (n = 5), Rac1 (n = 3) and Cdc42 (n = 3). Bars show SEM. * indicates significant difference found, p<0.05. C–D. F-actin in cells expressing Arhgap28-V5 (C) and Arhgap28R425A-V5 (D) was visualized by fluorescence microscopy using anti-V5 antibodies and Atto 488-conjugated phalloidin (representative images from 3 independent transfections). Arrows point to membrane ruffling and F-actin protrusions. Bars  = 25 µm.