Figure 6. Expression of Rho GAPs and Rho GTPases in Arhgap28^del bone tissues.

RNA was isolated from bone tissues (from tibia and fibula) of P0 wild type and Arhgap28^del mice. A and B. The expression of genes encoding Arhgap28 and related RhoGAPs, Arhgap6 and Arhgap18 (panel A); and Rho GTPases, RhoA, Rac1, Cdc42 and RhoQ (panel B) was quantified by qPCR (n = 3). Fold changes in gene expression normalized to Gapdh (2^−ΔΔCt values). Bars show SEM. *** and * indicate significant differences found when compared to wild type, p<0.001 and p<0.05, respectively.