Table 2. UCB-stem cell Class B (Profile-2) transcription factors.
Gene No. | GENE SYMBOL | ENRICHMENT SCORE1 | GENE RANK2 |
1 | SON | −0.05842792 | 2994 |
2 | TSC22D3 | −0.058295283 | 2927 |
3 | NFX1 | −0.054407794 | 2935 |
4 | DMC1 | −0.053078525 | 2844 |
5 | ATF3 | −0.051295283 | 2927 |
6 | TRIM22 | −0.04250992 | 3495 |
7 | SOX6 | −0.04150992 | 3495 |
8 | NFKB2 | −0.038754087 | 2935 |
9 | BATF | −0.03710674 | 3189 |
10 | GATA1 | −0.03329886 | 3749 |
11 | KLF1 | −0.032754087 | 2935 |
12 | FOXC1 | −0.030511508 | 3262 |
13 | SP140 | −0.03033415 | 3212 |
14 | STAT1 | −0.02901836 | 3336 |
15 | MAFB | −0.02863797 | 3306 |
16 | GLI2 | −0.02699749 | 3520 |
17 | BCL6 | −0.02135538 | 2685 |
18 | ZNF256 | −0.0191952 | 2182 |
19 | SYCP1 | −0.017439079 | 3545 |
20 | CDT1 | −0.017186195 | 2181 |
21 | E2F1 | −0.013077641 | 2999 |
22 | MXI1 | −0.012244189 | 3349 |
23 | IKZF4 | −0.008271581 | 2442 |
24 | HMGA1 | −0.007992041 | 2911 |
25 | CREB1 | −0.006540393 | 2653 |
26 | JUN | −0.005443786 | 3064 |
27 | OLIG2 | −0.003412304 | 3554 |
28 | SIAH2 | −0.002095686 | 3614 |
29 | HSF1 | −0.001728075 | 2562 |
30 | ZHX2 | −0.00001730 | 2292 |
ES, enrichment score of TFs identified by GSEA using three TF gene sets (TF activity, TF complex and DNA binding). A negative ES indicates genes with a negative correlation to Profile-1 (i.e. Profile-2 gene with increased expression from day 21 to day 56).
Gene Rank, gene rank list generated by GSEA using 3786 TFs with >1.5-fold changes in expression from day 21 to day 56 in culture.