Skip to main content
. 2014 Sep 11;9(9):e107284. doi: 10.1371/journal.pone.0107284

Table 1. Functional test results for immune competence in uninfected and Batrachochytrium dendrobatidis-exposed Litoria caerulea and L. infrafrenata following stimulation with intradermal phytohemagglutinin (PHA) and intracelomic sheep red blood cells.

Species Litoria caerulea Litoria infrafrenata
Status Healthy (n = 10) Infecteda (n = 10) Healthy (n = 10) Exposedb (n = 9)
Immune Parameter Mean SD Mean SD P value Mean SD Mean SD P value
Skin PHA Stimulation (mm) 0.14 0.46 0.04 0.08 0.495 0.25 0.39 −0.19 0.34 0.017
IgM + IgY Titre (LOG10) 4.7 3.1 3.7 1.3 0.370 4.9 2.3 4.6 2.7 0.800
IgY Titre (LOG10) 3.4c 2.5 4.1 4.2 0.529 4.0c 2.6 6.5 3.6 0.062
Splenic Cell Count (x106) 36.4 14.9 12.7 8.6 0.000 40.6 14.4 31.4 21.0 0.277
Splenic Cell Viability (%) 69.0 11.5 35.7 14.5 0.000 70.4 11.4 62.0 6.8 0.070
Rosette-forming Cells (x103) 2423 1369 898 629 0.005 855 497 755 356 0.623
Final BWd (g) 41.5 19.1 59.8 12.8 - 37.2 12.1 66.9 13.4 -
Kidney-BW Ratio 0.60 0.13 0.51 0.16 0.164 0.64 0.13 0.51 0.06 0.011
Liver-BW Ratio 5.23 1.61 2.89 0.64 0.001 3.23 0.65 3.39 1.16 0.726
Spleen-BW Ratio 0.04 0.01 0.04 0.01 0.216 0.13 0.07 0.12 0.06 0.555
a

100% of 10 exposed L. caerulea were infected 75 d post-exposure to Bd.

b

0% of 9 exposed L. infrafrenata were infected 75 d post-exposure to Bd.

c

n = 9.