Table 1. Functional test results for immune competence in uninfected and Batrachochytrium dendrobatidis-exposed Litoria caerulea and L. infrafrenata following stimulation with intradermal phytohemagglutinin (PHA) and intracelomic sheep red blood cells.
Species | Litoria caerulea | Litoria infrafrenata | ||||||||
Status | Healthy (n = 10) | Infecteda (n = 10) | Healthy (n = 10) | Exposedb (n = 9) | ||||||
Immune Parameter | Mean | SD | Mean | SD | P value | Mean | SD | Mean | SD | P value |
Skin PHA Stimulation (mm) | 0.14 | 0.46 | 0.04 | 0.08 | 0.495 | 0.25 | 0.39 | −0.19 | 0.34 | 0.017 |
IgM + IgY Titre (LOG10) | 4.7 | 3.1 | 3.7 | 1.3 | 0.370 | 4.9 | 2.3 | 4.6 | 2.7 | 0.800 |
IgY Titre (LOG10) | 3.4c | 2.5 | 4.1 | 4.2 | 0.529 | 4.0c | 2.6 | 6.5 | 3.6 | 0.062 |
Splenic Cell Count (x106) | 36.4 | 14.9 | 12.7 | 8.6 | 0.000 | 40.6 | 14.4 | 31.4 | 21.0 | 0.277 |
Splenic Cell Viability (%) | 69.0 | 11.5 | 35.7 | 14.5 | 0.000 | 70.4 | 11.4 | 62.0 | 6.8 | 0.070 |
Rosette-forming Cells (x103) | 2423 | 1369 | 898 | 629 | 0.005 | 855 | 497 | 755 | 356 | 0.623 |
Final BWd (g) | 41.5 | 19.1 | 59.8 | 12.8 | - | 37.2 | 12.1 | 66.9 | 13.4 | - |
Kidney-BW Ratio | 0.60 | 0.13 | 0.51 | 0.16 | 0.164 | 0.64 | 0.13 | 0.51 | 0.06 | 0.011 |
Liver-BW Ratio | 5.23 | 1.61 | 2.89 | 0.64 | 0.001 | 3.23 | 0.65 | 3.39 | 1.16 | 0.726 |
Spleen-BW Ratio | 0.04 | 0.01 | 0.04 | 0.01 | 0.216 | 0.13 | 0.07 | 0.12 | 0.06 | 0.555 |
100% of 10 exposed L. caerulea were infected 75 d post-exposure to Bd.
0% of 9 exposed L. infrafrenata were infected 75 d post-exposure to Bd.
n = 9.