Table 2. Pre- and post-immune stimulation hematologic values for uninfected and Batrachochytrium dendrobatidis-infected Litoria caerulea.
Status | Uninfected (n = 10) | Infected (n = 10) | |||||||||
Immune Stimulation | Pre (day 0) | Post (day 7) | Pre (day 0) | Post (day 7) | |||||||
Parameter | Mean | SD | Mean | SD | P valuea | Mean | SD | Mean | SD | P valuea | P valueb |
PCV (%) | 35.9 | 3.4 | 28.9 | 4.8 | 0.004 | 36.1 | 4.0 | 34.5 | 7.5 | 0.485 | 0.074 |
Hb (g dl−1) | 8.7 | 1.2 | 6.2 | 1.4 | 0.000 | 9.0 | 2.4 | 8.6 | 1.8 | 0.670 | 0.042 |
RBC (x109 l−1) | 694 | 135 | 588 | 120 | 0.137 | 553 | 90 | 580 | 132 | 0.515 | 0.098 |
MCV (fl) | 536 | 117 | 503 | 107 | 0.402 | 663 | 91 | 606 | 107 | 0.237 | 0.697 |
MCH (pg) | 129 | 25 | 107 | 24 | 0.021 | 165 | 44 | 151 | 26 | 0.493 | 0.675 |
MCHC (g l−1) | 245 | 38 | 214 | 30 | 0.010 | 247 | 46 | 252 | 33 | 0.827 | 0.137 |
Thrombocyte (×109 l−1) | 34.8 | 9.0 | 30.5 | 7.3 | 0.299 | 27.0 | 5.9 | 23.6 | 4.0 | 0.042 | 0.823 |
WBC (x109 l−1) | 24.6 | 8.9 | 39.7 | 15.4 | 0.026 | 6.8 | 1.9 | 8.5 | 4.0 | 0.175 | 0.044 |
Neutrophil (×109 l−1) | 3.9 | 1.8 | 4.9 | 2.2 | 0.303 | 2.0 | 1.0 | 2.3 | 1.8 | 0.576 | 0.513 |
Lymphocyte (×109 l−1) | 19.0 | 7.1 | 31.2 | 13.9 | 0.026 | 4.1 | 1.5 | 5.0 | 1.9 | 0.179 | 0.035 |
Neut-lymph ratio | 0.22 | 0.10 | 0.18 | 0.09 | 0.224 | 0.54 | 0.32 | 0.46 | 0.24 | 0.376 | 0.694 |
Monocyte (×109 l−1) | 1.3 | 0.9 | 3.1 | 1.7 | 0.005 | 0.6 | 0.7 | 1.1 | 0.7 | 0.115 | 0.038 |
Eosinophil (×109 l−1) | 0.50 | 0.95 | 0.50 | 0.68 | 0.814 | 0.06 | 0.08 | 0.10 | 0.12 | 0.055 | 0.676 |
Basophil (×109 l−1) | 0.00 | 0.00 | 0.04 | 0.12 | 0.343 | 0.00 | 0.00 | 0.00 | 0.00 | - | 0.343 |
Polychromasia (%) | 6.9 | 4.0 | 13.8 | 10.5 | 0.044 | 3.3 | 1.8 | 2.6 | 2.8 | 0.396 | 0.032 |
BW, body weight.
Paired-samples t-tests between days 0 and 7 within each group (uninfected and infected).
Independent-samples t-tests between the two groups for the change in each variable from day 0 to day 7.