Table 3. Pre- and post-immune stimulation plasma biochemical and serum protein electrophoretic values for uninfected and Batrachochytrium dendrobatidis-infected Litoria caerulea.

Status	Uninfected (n = 10)					Infected (n = 10)
Immune Stimulation	Pre (day 0)		Post (day 7)			Pre (day 0)		Post (day 7)
Parameter	Mean	SD	Mean	SD	P valuea	Mean	SD	Mean	SD	P valuea	P valueb
AST (U l−1)	69	37	73	34	0.800	68	14	97	42	0.068	0.268
CK (U l−1)	495	344	636	496	0.566	282	185	555	337	0.074	0.632
Uric Acid (µmol l−1)	37	27	40	22	0.813	35	18	37	29	0.811	0.937
Glucose (mmol l−1)	3.5	0.8	3.4	0.7	0.766	4.5	0.9	4.6	0.9	0.715	0.640
Calcium (mmol l−1)	3.03	0.46	3.00	0.54	0.861	2.75	0.40	2.72	0.40	0.697	0.936
Phosphorus (mmol l−1)	1.70	0.46	1.49	0.42	0.194	0.88	0.23	0.83	0.35	0.587	0.389
Ca-P ratio	1.89	0.58	2.16	0.59	0.193	3.29	0.82	3.78	1.42	0.260	0.639
Potassium (mmol l−1)	4.8	1.7	4.2	1.9	0.288	5.2	1.5	4.2	2.2	0.343	0.749
Sodium (mmol l−1)	111.7	6.1	111.5	4.8	0.929	109.5	6.2	108.5	4.9	0.535	0.768
Total Protein (g l−1)	57.4	6.3	62.6	6.8	0.106	57	7	54	6	0.353	0.069
A-G ratio	0.57	0.12	0.44	0.09	0.008	0.46	0.16	0.54	0.17	0.000	0.000
Albumin (g l−1)	20.5	3.4	18.7	2.2	0.130	18	5	18	4	0.604	0.106
Total globulins (g l−1)	36.9	5.2	43.9	6.6	0.024	39.2	4.9	35.8	6.6	0.207	0.010
α-1 globulin (g l−1)	22.7	4.1	28.0	4.0	0.026	21.5	3.6	20.7	5.2	0.620	0.030
α-2 globulin (g l−1)	6.9	1.2	8.2	3.1	0.302	9.2	1.5	7.9	2.0	0.092	0.072
β globulin (g l−1)	5.2	1.6	4.9	1.5	0.696	5.8	1.7	5.0	1.0	0.077	0.541
γ globulin (g l−1)	2.2	0.8	2.8	1.1	0.023	2.7	1.0	2.2	0.7	0.072	0.004

^aPaired-samples t-tests between days 0 and 7 within each group (uninfected and infected).

^bIndependent-samples t-tests between the two groups for the change in each variable from day 0 to day 7.