Table 4. Pre- and post-immune stimulation hematologic values for uninfected and Batrachochytrium dendrobatidis-exposed but uninfected Litoria infrafrenata.
| Status | Uninfected (n = 10) | Exposed uninfected (n = 9) | |||||||||
| Immune Stimulation | Pre (day 0) | Post (day 7) | Pre (day 0) | Post (day 7) | |||||||
| Parameter | Mean | SD | Mean | SD | P valuea | Mean | SD | Mean | SD | P valuea | P valueb |
| PCV (%) | 26.9 | 7.0 | 25.4 | 4.1 | 0.548 | 35.7 | 6.0 | 32.3 | 4.8 | 0.079 | 0.548 |
| Hb (g d l−1) | 7.1 | 2.5 | 5.6 | 1.1 | 0.086 | 8.6 | 1.3 | 7.4 | 1.7 | 0.024 | 0.691 |
| RBC (x109 l−1) | 736 | 208 | 630 | 129 | 0.048 | 783 | 109 | 662 | 108 | 0.018 | 0.811 |
| MCV (fl) | 383 | 155 | 421 | 124 | 0.450 | 459 | 74 | 500 | 121 | 0.377 | 0.969 |
| MCH (pg) | 107 | 60 | 93 | 32 | 0.525 | 111 | 19 | 116 | 38 | 0.674 | 0.457 |
| MCHC (g l−1) | 291 | 126 | 220 | 29 | 0.142 | 245 | 41 | 232 | 47 | 0.306 | 0.240 |
| Thrombocyte (×109 l−1) | 54.8 | 12.9 | 27.2 | 7.2 | 0.000 | 33.7 | 10.1 | 26.8 | 7.0 | 0.036 | 0.001 |
| WBC (x109 l−1) | 32.9 | 8.7 | 47.6 | 10.2 | 0.005 | 33.7 | 27.0 | 31.0 | 17.4 | 0.616 | 0.015 |
| Neutrophil (×109 l−1) | 5.8 | 5.9 | 16.4 | 5.1 | 0.002 | 12.1 | 14.3 | 9.3 | 10.0 | 0.204 | 0.001 |
| Lymphocyte (×109 l−1) | 24.3 | 7.7 | 25.5 | 6.2 | 0.726 | 17.8 | 11.1 | 17.3 | 11.1 | 0.860 | 0.700 |
| Neut-lymph ratio | 0.28 | 0.36 | 0.67 | 0.24 | 0.038 | 0.66 | 0.47 | 0.71 | 0.71 | 0.726 | 0.120 |
| Monocyte (×109 l−1) | 2.1 | 1.4 | 5.6 | 3.5 | 0.023 | 3.3 | 3.9 | 4.0 | 3.1 | 0.522 | 0.131 |
| Eosinophil (×109 l−1) | 0.25 | 0.44 | 0.04 | 0.13 | 0.205 | 0.57 | 0.76 | 0.34 | 0.45 | 0.355 | 0.925 |
| Basophil (×109 l−1) | 0.47 | 0.79 | 0.00 | 0.00 | 0.095 | 0.00 | 0.00 | 0.00 | 0.00 | - | 0.095 |
| Polychromasia (%) | 5.5 | 3.6 | 15.3 | 7.9 | 0.004 | 9.3 | 5.1 | 11.1 | 6.7 | 0.108 | 0.013 |
a
Paired-samples t-tests between days 0 and 7 within each group (uninfected and exposed).
b
Independent-samples t-tests between the two groups for the change in each variable from day 0 to day 7.