Table 5. Pre- and post-immune stimulation plasma biochemical and serum protein electrophoretic values for uninfected and Batrachochytrium dendrobatidis-exposed but uninfected Litoria infrafrenata.

Status	Uninfected (n = 10)					Exposed uninfected (n = 9)
Immune Stimulation	Pre (day 0)		Post (day 7)			Pre (day 0)		Post (day 7)
Parameter	Mean	SD	Mean	SD	P valuea	Mean	SD	Mean	SD	P valuea	P valueb
AST (U l−1)	248	406	171	93	0.498	100	52	127	84	0.309	0.390
CK (U l−1)	556	286	1137	560	0.013	918	864	1276	929	0.218	0.539
Uric Acid (µmol l−1)	8	17	24	37	0.251	3.8	3.4	5.3	6.6	0.433	0.302
Glucose (mmol l−1)	3.6	0.6	2.9	0.8	0.036	3.4	0.4	4.2	1.0	0.020	0.002
Calcium (mmol l−1)	2.29	2.09	2.38	0.99	0.827	2.90	0.64	3.01	0.80	0.628	0.956
Phosphorus (mmol l−1)	1.54	0.63	1.73	0.76	0.404	1.59	0.43	1.52	0.32	0.412	0.279
Ca-P ratio	1.38	0.49	1.47	0.40	0.608	1.88	0.36	2.01	0.45	0.388	0.841
Potassium (mmol l−1)	3.7	0.8	3.8	0.9	0.591	4.8	2.0	4.2	1.5	0.183	0.145
Sodium (mmol l−1)	107.7	2.7	110.9	4.1	0.065	110.0	3.6	108.7	3.1	0.336	0.138
Total Protein (g l−1)	29	6	34	7	0.007	40	8	38	5	0.500	0.065
A-G ratio	0.11	0.04	0.13	0.04	0.033	0.21	0.06	0.23	0.07	0.175	0.609
Albumin (g l−1)	2.9	1.1	3.9	1.4	0.013	7.0	2.7	6.9	1.0	0.866	0.123
Total globulins (g l−1)	26.1	6.1	30.5	5.8	0.016	33.2	5.9	31.0	5.0	0.470	0.070
α-1 globulin (g l−1)	9.2	2.1	11.6	4.2	0.041	11.6	2.0	9.3	3.0	0.057	0.004
α-2 globulin (g l−1)	5.3	1.7	6.8	2.9	0.418	6.1	2.6	5.7	2.3	0.654	0.079
β globulin (g l−1)	10.1	5.5	11.0	4.0	0.192	13.7	4.2	14.6	2.7	0.665	0.968
γ globulin (g l−1)	1.4	0.8	1.2	0.3	0.271	1.3	0.4	1.4	0.3	0.883	0.317

^aPaired-samples t-tests between days 0 and 7 within each group (uninfected and exposed).

^bIndependent-samples t-tests between the two groups for the change in each variable from day 0 to day 7.