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. 2014 Sep 11;10(9):e1004355. doi: 10.1371/journal.ppat.1004355

Figure 5. VP4 fusion-loop mutant and VP7 disulfide-linked trimer.

Figure 5

A. Effects of mutations on uncoating. Particles recoated with VP4 fusion-loop mutant (top panel) and VP7 disulfide-linker trimer (bottom panel) added to BSC-1 cells and imaged at 2 min and 45 min. All particles in the field at 45 sec retain VP7. B. Percent uncoating for particles recoated with wt proteins, with VP4 mutant and VP7 wt, and with VP4 wt and VP7 mutant. Average of three experiments; bars show standard error. C. Percent of particles protected from EDTA dissociation (for wt and VP4-391D) and from access by m159 (for VP7 C-C) as a function of time. Average of three experiments; bars show standard error.