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. 2014 Sep 11;10(9):e1004364. doi: 10.1371/journal.ppat.1004364

Figure 6. Recombinant CBMs and LacNAc and/or lactose reduce pneumococcal adherence in a BgaA-dependent manner.

Figure 6

(A) Adherence of S. pneumoniae strain R6 and R6ΔbgaA to NHBE cells in the presence of CBM71-1, CBM71-2 or CBM71-1.2 (250 µM). Asterisks indicate significant differences in adherence in the presence or absence of CBM. (B) Adherence of S. pneumoniae strain C06_18 and C06_18ΔbgaA to NHBE cells in the presence of CBM71-1, CBM71-2 or CBM71-1.2 (250 µM). (C) Adherence of S. pneumoniae strain R6 and R6ΔbgaA to NHBE cells in the presence of LacNAc and lactose (0–10 mM). Asterisks indicate significant differences in adherence in the presence or absence of disaccharide. (D) Adherence of S. pneumoniae strain C06_18 and C06_18ΔbgaA to NHBE cells in the presence of LacNAc and lactose (0–10 mM). As in (C) except using pneumococcal strain C06_18. Adherence assays are mean ± SD of three independent experiments each performed in triplicate. Statistically significant differences were assessed using a two-tailed Student's t-test. * p≤0.04, ** p≤0.007, *** p≤8×10−4.