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. 2014 Sep 11;9(9):e107611. doi: 10.1371/journal.pone.0107611

Figure 1. Up-regulation of Wnt5a, Fzd2, and Ror2 mRNAs in the X. laevis intestine during natural (A) and T3-induced metamorphosis (B).

Figure 1

Total RNA was prepared from the intestine of tadpoles at indicated developmental stages (A) or stage 54-tadpoles after 10 nM T3 treatment (B), and was analyzed by qRT-PCR. The level of each mRNA is shown relative to the level of ribosomal protein L8 (rpL8) mRNA, with the values at stage 54 or day 0 of T3 treatment set to 1. Error bars represent the SEM (n = 4). Asterisks indicate that the transcription is significantly up- or down-regulated between the indicated stages or days after T3 treatment. *: P<0.05; **: P<0.01; ns: not significant.