Table 2. Constructed mutants and plasmids utilised in this study.
| Strain | CDS annotation | Locus | Insertion site | Mutant | Description |
| C. botulinum (ATCC3502) | gerXA1 | CBO0123 | 42s | gerXA1-0123− | Single insertional knockout |
| gerXA2 | CBO1975 | 56s | gerXA2-1975− | Single insertional knockout | |
| gerXA3 | CBO2797 | 1344a | gerXA3-2797− | Single insertional knockout | |
| C. sporogenes (ATCC15579) | gerXA1 | CLOSPO_00838 | 175s | gerXA1-00838− | Single insertional knockout |
| gerXA2 | CLOSPO_03006 | 795s | gerXA2-03006− | Single insertional knockout | |
| gerXA3 | CLOSPO_02217 | 61s | gerXA3-02217− | Single insertional knockout | |
| gerXA4 | CLOSPO_02140 | 601s | gerXA4-02140− | Single insertional knockout | |
| gerXA1, | CLOSPO_00838, | 175s, | gerXA 3−02140+ | Triple insertional knockout leaving one functional GerXA gerXA4 | |
| gerXA2, | CLOSPO_03006, | 795s, | |||
| gerXA3 | CLOSPO_02217 | 61s | |||
| gerXA1, | CLOSPO_00838, | 175s, | gerXA 3−02217+ | Triple insertional knockout leaving one functional GerXA gerXA3 | |
| gerXA2, | CLOSPO_03006, | 795s, | |||
| gerXA4 | CLOSPO_02140 | 601s | |||
| gerXA1, | CLOSPO_00838, | 175s, | gerXA 3−03006+ | Triple insertional knockout leaving one functional GerXA gerXA2 | |
| gerXA3, | CLOSPO_02217, | 61s, | |||
| gerXA4 | CLOSPO_02140 | 601s | |||
| gerXA2, | CLOSPO_03006, | 795s, | gerXA 3−00838+ | Triple insertional knockout leaving one functional GerXA gerXA1 | |
| gerXA3, | CLOSPO_02217, | 61s, | |||
| gerXA4 | CLOSPO_02140 | 601s | |||
| gerXA1, | CLOSPO_00838, | 175s, | gerXA 4− | Quadruple insertional knockout. Insertion in all known gerXA sub-units | |
| gerXA2, | CLOSPO_03006, | 795s, | |||
| gerXA3, | CLOSPO_02217, | 61s, | |||
| gerXA4 | CLOSPO_02140 | 601s | |||
| Plasmids | pMTL007C-E2 | Clostridial mutagenesis plasmid | |||
| pMTL8315esp | GR expression plasmid with no fdx promoter. Relies on native promoter of inserted genes | ||||
| pMTL8315fdx | GR expression plasmid with fdx promoter |
− indicates the gene is absent; + indicates that only that gene is functional; no− describes the number of genes knocked out. a = antisense orientation insertion site; s = sense orientation insertion site. The designation of “X” before the letter A is used in clostridia as unlike Bacillus, GRs in these species have not yet been attributed to specific germinants.