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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Adv Mater. 2014 Jul 22;26(33):5815–5822. doi: 10.1002/adma.201400941

Figure 4. Assessment of RBC functional properties following ectoine-based vitrification and rewarming.

Figure 4

a–d Functional properties for fresh RBCs (Control, solid line) and recovered RBCs after ectoine-based vitrification (EV, dotted line) were compared. Four types of characteristic RBC function were measured: (a) determined band-3 phosphorylation level, (b) CD35 expression, (c) intracellular nitric oxide (NO) level, and (d) intracellular reactive oxygen species (ROS) level. The negative controls (unstained fresh RBCs represented by the gray shaded region and unstained recovered RBCs after EV represented by the dashed line) were not labeled and used as a baseline to detect the auto-fluorescence of cells. The measurements were performed following incubation of RBCs with 25% serum from a universal acceptor for 30 mins at 37 C°.