Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Sep 15;25(18):2761–2773. doi: 10.1091/mbc.E14-05-0966

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Britigan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 1: — ARF loss causes aneuploidy in vitro. (A) Diploid MEF chromosome spread with 40 chromosomes. Scale bar, 10 μm. (B) Average aneuploidy in MEFs. n = 3 experiments of 50 spreads each. (C) Histogram of chromosome spreads from B. Inset, enlarged histogram showing the percentages of near-tetraploid MEFs. (D) Tetraploid MEF chromosome spread with 80 chromosomes. Scale bar, 10 μm. (E) Average tetraploidy in MEFs. n = 3 experiments of 100 spreads each. (F) Binucleate MEF. Red, F-actin stained with phalloidin. Blue, DNA. Scale bar, 20 μm. (G) Average percentage of binucleation in MEFs of the indicated genotypes. n = 250 cells from each of three independent experiments. **p < 0.001.