FIGURE 2:

ARF is required to maintain chromosomal stability in vivo. (A) Chromosome spread from a mouse splenocyte with 40 chromosomes. Scale bar, 10 μm. (B) Average aneuploidy in 5-mo-old mouse splenocytes. n = 3 independent experiments of 50 spreads each. (C) Histogram of chromosome spreads from B. No tetraploid spreads were observed in splenocytes. Mitotic indices of splenocytes were comparable in wild-type (0.52 ± 0.18%) and ARF-null (0.62 ± 0.03%) animals. n ≥ 930 cells from three animals. (D) Murine intestine labeled with FISH probe to chromosome 11. Top right is labeled to indicate nuclear boundaries and number of chromosome 11 signals in each cell. (E) Percentage of cells of the indicated genotypes with greater or less than two copies of chromosome 11. (F) Percentage of cells with the indicated number of copies of chromosome 11. *p < 0.05; **p < 0.001.