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. 2014 Sep 15;25(18):2761–2773. doi: 10.1091/mbc.E14-05-0966

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© 2014 Britigan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Mitotic functions of ARF are p53-independent. (A) Percentage of MEFs with misaligned chromosomes in metaphase. Loss of ARF increases the incidence of misaligned chromosomes in p53^−/− cells. n > 80 metaphases from three independent experiments. (B) Percentage of MEFs with lagging chromosomes in anaphase or telophase. Although loss of either ARF or p53 increases the percentage of cells containing lagging chromosomes, reduction of ARF in p53^−/− cells causes a further increase, consistent with a p53-independent role for ARF in preventing chromosome lagging. n > 100 anaphases and telophases from each of three independent experiments. (C) Mitotic index of MEFs of the indicated genotypes ± 16 h of colcemid treatment. ARF, but not p53, is required for the mitotic checkpoint–mediated increase in mitotic index in response to colcemid. n > 250 cells from each of three independent experiments. *p < 0.05.