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. 2014 Sep 15;25(18):2853–2865. doi: 10.1091/mbc.E14-05-0971

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© 2014 Bone et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Mutations in the nucleoplasmic domain of UNC-84 lead to an intermediate nuclear migration defect. (A) Cartoon describing hyp7 precursor nuclear migration on the dorsal surface of the pre–comma-stage embryo. In wild-type embryos (top), two rows of hyp7 precursors (gray) intercalate to form a row of column-shaped cells. Nuclei then migrate from right to left (green) or left to right (purple). In unc-84(null) mutant embryos, intercalation occurs normally, but the nuclei fail to migrate. Instead, underlying body wall muscle migrations push unc-84 nuclei to the dorsal cord (arrow). The dorsal surface is shown; anterior is left. (B) Average number of nuclei present in the dorsal cord of L1 larvae, which approximates the number of failed nuclear migrations. Error bars, 95% CI. (C–G) The number of nuclei in the larval dorsal cord was counted following hypodermal nuclei that express a nucleoplasmic GFP from ycIs10[p_col-10nls::gfp::lacZ]. Lateral views of L1 larvae. Dorsal is up, and anterior is left; the dorsal cord (arrow in A) is demarcated by the white dotted line. Scale bar, 10 μm. Representative images of (C) wild type, (D) unc-84(null), (E) unc-84(P91S), (F) unc-84(Δ40-161), and (G) unc-84(Δ1-208). (H) Schematic of the domain structure of UNC-84. The conserved SUN domain is red, and the transmembrane span is black. The mutants discussed in the text are indicated.