FIGURE 2:

UNC-84 and LMN-1 interact in a yeast two-hybrid assay. (A) Yeast growing in a directed yeast two-hybrid assay. All yeast express the LMN-1::Gal4AD prey construct and the UNC-84::Gal4BD bait construct indicated on the left. Yeast were grown to the same concentration, serially diluted (as indicated at the top), and plated on SD−Trp−Leu−His medium, which requires an interaction to grow (left), or SD−Trp−Leu medium as control (right). (B) Activity of the lacZ gene as activated by a liquid o-nitrophenyl-β-galactoside assay that represents a two-hybrid interaction. Average β-galactosidase units (ΔOD₄₂₀/min/ml of cells) from three different experiments, each done in triplicate, and the associated 95% CI error bars. Significant statistical differences as determined by Student's t test are noted at the top.