FIGURE 2:

NFAT5 regulates REDD1 expression in HCT116, SW480 and Caco-2 cells. (A) HCT116, SW480, and Caco-2 cells were transfected with NFAT5 or NTC siRNA. After 48-h incubation, transfected cells were lysed, and Western blot analysis was performed using antibodies against REDD1, p-Ser-6, Ser-6, NFAT5, and β-actin. (B) HCT116 and Caco-2 cells were transiently transfected with empty vector or a construct expressing Myc-NFAT5. Forty-eight hours after transfection, cells were lysed, and Western blot analysis was performed using antibodies against REDD1, Myc-tag, and β-actin. REDD1 signals from three separate experiments were quantitated densitometrically and expressed as fold change with respect to β-actin.