Figure 1. Number of CD4⁺ and CD8⁺ T cells in the BAL of IRD mice.

Cells were recovered from the lungs of Pneumocystis carinii infected SCID mice by BAL at various times following immune reconstitution. The percentage of CD4⁺ and CD8⁺ T cells present in the BAL was then determined by FACS analysis, and were used to calculate numbers of CD4⁺ or CD8⁺ T cells from total cell counts. Closed circles represent CD4⁺ T cells and open circles represent CD8⁺ T cells. Values in bracket above each CD4⁺ time point shows Pneumocystis carinii burden expressed as Log10(kexin DNA copies) at that particular data point as assessed by quantitative real-time PCR and was measured as described in Materials and Methods. Each data point is expressed as the arithmetic mean ± 1 SEM (n= 4-7 mice at each time point; * denotes p≤0.05).