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. Author manuscript; available in PMC: 2014 Sep 11.
Published in final edited form as: Am J Physiol Lung Cell Mol Physiol. 2006 Aug 4;291(6):L1256–L1266. doi: 10.1152/ajplung.00079.2006

Figure 6. CD4+ IFN-γ+ T cells are readily detected in the lung tissue of experimental mice.

Figure 6

Lung cells were isolated from experimental mice sacrificed on the seventh day after immune reconstitution, stimulated with PMA and ionomycin, and stained with fluorescent MAbs against the following surface markers: CD4 (APC) and CD8 (PerCP) as well as intracellular IFN-γ (FITC). Results of pooled cells from CD8-depleted IRD mice are shown in Panel A. The same cells pre-treated with 10 µg of unlabeled anti-IFN-γ shows nearly complete blocking of IFN staining. The percentage of CD4+ IFN-γ+ cells [cells in the gate R2] from a similar plot for each sample were used to calculate total number of IFN-γ -producing CD4+ T cells in that sample, and the results are shown in Panel C. Bars represent arithmetic means of three samples and error bars represent 1 SEM *p<0.05..