Table 1.
Effects of morbid obesity on femoral bone mineral content and density, femoral cortical and cancellous bone microarchitecture, and vertebral cancellous bone microarchitecture in leptin-deficient ob/ob mice.
| End Point | WT | ob/ob | ob/ob (pair-fed) | ANOVA P* |
|---|---|---|---|---|
| Dual Energy X-ray Absorptiometry | ||||
| Total Femur | ||||
| BMC (g) | 0.023 ± 0.000 | 0.019 ± 0.000a | 0.017 ± 0.000a,b | 0.0001 |
| Bone area (cm2) | 0.43 ± 0.01 | 0.40 ± 0.00a | 0.37 ± 0.01a,b | 0.0001 |
| BMD (g/cm2) | 0.053 ± 0.001 | 0.048 ± 0.000a | 0.047 ± 0.000a | 0.0003 |
| Microcomputed Tomography | ||||
| Total Femur | ||||
| Length (mm) | 14.9 ± 0.1 | 14.2 ± 0.1a | 13.4 ± 0.1a,b | <0.0001 |
| Bone volume (mm3) | 18.5 ± 0.3 | 16.8 ± 0.3a | 15.0 ± 0.3a,b | <0.0001 |
| Midshaft Femur (cortical bone) | ||||
| Cross-sectional volume (mm3) | 0.41 ± 0.01 | 0.40 ± 0.01 | 0.42 ± 0.01 | 0.4220 |
| Cortical volume (mm3) | 0.20 ± 0.00 | 0.18 ± 0.00a | 0.17 ± 0.00a,b | 0.0008 |
| Marrow volume (mm3) | 0.22 ± 0.00 | 0.23 ± 0.00 | 0.25 ± 0.01a,b | 0.0012 |
| Cortical thickness (μm) | 195 ± 3 | 176 ± 3a | 159 ± 2a,b | 0.0001 |
| Polar moment of inertia (mm4) | 0.36 ± 0.01 | 0.33 ± 0.01 | 0.33 ± 0.01 | 0.4220 |
| Distal Femur Metaphysis (cancellous bone) | ||||
| Bone volume/tissue volume (%) | 11.2 ± 0.8 | 13.2 ± 0.5a | 18.1 ± 0.5a,b | 0.0004 |
| Connectivity density (1/mm3) | 106 ± 9 | 173 ± 7a | 190 ± 8a | <0.0001 |
| Trabecular thickness (μm) | 44 ± 1 | 41 ± 1 | 45 ± 0b | 0.0365 |
| Trabecular number (mm−1) | 4.8 ± 0.1 | 5.2 ± 0.1a | 5.7 ± 0.1a,b | 0.0001 |
| Trabecular separation (μm) | 210 ± 4 | 191 ± 4a | 173 ± 4a,b | 0.0013 |
| Distal Femur Epiphysis (cancellous bone) | ||||
| Bone volume/tissue volume (%) | 31.2 ± 0.6 | 36.1 ± 0.6a | 36.6 ± 0.9a | 0.0013 |
| Connectivity density (1/mm3) | 155 ± 4 | 254 ± 8a | 261 ± 7a | <0.0001 |
| Trabecular thickness (μm) | 64 ± 1 | 60 ± 1a | 58 ± 1a | 0.0001 |
| Trabecular number (mm−1) | 5.5 ± 0.1 | 6.7 ± 0.1a | 6.7 ± 0.1a | 0.0004 |
| Trabecular separation (μm) | 179 ± 2 | 138 ± 3a | 138 ± 3a | <0.0001 |
| Lumbar Vertebra (cancellous bone) | ||||
| Bone volume/tissue volume (%) | 21.8 ± 1.0 | 31.5 ± 0.8a | 34.2 ± 0.5a,b* | <0.0001 |
| Connectivity density (1/mm3) | 198 ± 10 | 364 ± 15a | 191 ± 5b | <0.0001 |
| Trabecular thickness (μm) | 49 ± 1 | 50 ± 0.5 | 54 ± 0.4a,b | 0.0010 |
| Trabecular number (mm−1) | 4.6 ± 0.1 | 6.0 ± 0.1a | 5.8 ± 0.1a | 0.0004 |
| Trabecular separation (μm) | 215 ± 5 | 160 ± 3a | 163 ± 3a | 0.0005 |
Data are mean ± SE
*
The Hommel method for maintaining the family-wise error rate at 5% was used to adjust for multiple comparisons
a
Different from WT, p < 0.05
b
Different from ob/ob, p < 0.05