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. 2014 Aug 28;3:478. doi: 10.1186/2193-1801-3-478

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© Liu et al.; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Carotenoid biosynthesis pathway in Oncidium orchid and analysis of the expression level of OgPSY in transgenic Oncidium . (A) The scheme of carotenoid biosynthesis pathway in Oncidium orchid. *Indicates the target gene of PSY for RNA interference-based gene silencing. (B) Relative transcript level of OgPSY gene in wild type and OgPSY RNAi transgenic Oncidium orchid. GGDS: GGPP synthase; PSY: phytoene synthase; PDS: phytoene desaturase; ZDS: zeaxanthin desaturase; LCY: lycopene cyclase. ZEP: zeaxanthin epoxidase. Gene transcript levels were measured by RT-qPCR. Transcript levels were calculated and normalized with respect to ubiquitin mRNA. The experiment was repeated three times.