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. 2014 Sep 4;7:420. doi: 10.1186/1756-3305-7-420

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© Mouttaki et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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2.5% Agarose gel electrophoresis of ITS1-PCR restriction fragments. Wells: 1 – 10: patient samples; L. m: reference DNA of L. major; L. t: reference DNA of L. tropica; L. i: reference DNA of L. infantum; NC: negative control sample (H2O); WM: Weight Marker (50 bp DNA Ladder; Invitrogen Life Technologies, Brazil).