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. 2014 Sep 3;13:120. doi: 10.1186/s12934-014-0120-5

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© Ruth et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Screening results of improved carboxylesterase-AFT1 P. pastoris strains. Secretion levels: determined by ELISA and normalized to the respective starting strain. Starting strains: CE#12 (blue, low level expressing strain), CE#18 (grey, high level expressing strain). AFT1-A and AFT1-B: AFT1 overexpressing clones.