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. 2014 Sep 4;10:55. doi: 10.1186/1744-8069-10-55

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© Walder et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Determination of transcript levels (Cq) of the candidate reference genes in the dorsal horn of the spinal cord (A, B) and RVM (C, D). Levels were assessed four days (A, C) or two weeks (B, D) after intraplantar injection of CFA or saline. Values are the mean ± SEM of determinations made in 6–7 rats. In the dorsal horn, no differences were observed in the transcripts levels between saline- and CFA-treated rats, or between four days and two weeks, for any reference gene. In the RVM, transcript levels for each reference gene did not differ between saline- and CFA-treated rats. Although the Cq values at two weeks were statistically different from values at four days, the magnitude of the difference was about 0.2 Cq and unlikely to be biologically significant.