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. 2014 Sep 4;10:55. doi: 10.1186/1744-8069-10-55

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© Walder et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Determination of the optimal number of reference genes by the NormFinder algorithm. Stepwise calculation of stability began with the most stable gene (Mapk6), followed by serial addition of the next most stable gene and calculation of the accumulated standard deviation (circles). In both the dorsal horn (A) and in the RVM (B), the optimal number of reference genes to be used in qPCR experiments was three (open circle).