Fig. 1.

Oatp1 expression enhances bioluminescent output of luciferase-expressing cells. a HEK 293T cells expressing luciferase were transduced with a lentiviral vector expressing either Oatp1 or Timd2 (control) or were not transduced (control), 24 h before incubation with luciferin. Oatp1-expressing cells emitted significantly more light than control cells immediately after luciferin addition (1-s exposure time; n = 3, two-tailed T test). b Lewis lung carcinoma cells (LL2) expressing luciferase were transduced with lentiviral vectors, as described in (a). Clonal HEK 293T cells expressing Oatp1 produce more light than the luciferase-expressing cell population from which they were derived, for a period of 20 min following addition of luciferin at a concentration of c 36 μΜ and d 535 μM (n = 3, error bars show SD). e Western blot of protein extracts from the cells used in c and d, showing luciferase-YFP and RFP expression in control HEK 293T cells and HEK 293T cells transduced to express Oatp1. The presence of RFP indicates expression of the mStrawberry-Oatp1 transgene. sr steradian.