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. 2014 Sep 12;4:6344. doi: 10.1038/srep06344

Figure 4. PCA impairs the downstream activations of GPCR signaling.

Figure 4

(A) Activation profiles of MAPK signaling proteins in HeLa cells in the presence or absence of 100 μM PCA. Cells were incubated with serum-free media for 12 hr, followed by serum-containing media. Samples were collected at the indicated time points and analyzed by SDS-PAGE/IB. (B & C) Quantification of phospho-MEK1 (B) and phospho-ERK1/2 (C) normalized by total MEK1 and ERK1/2, respectively, in the presence/absence of PCA. Multiple film images were quantified by densitometry and data represent mean ± SD (n = 3). (D) As in (A) except that the experiment was performed using wild-type (+/+) and ATE1-/- MEFs. Cropped gels/blots are used in Western blot data.