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. Author manuscript; available in PMC: 2014 Sep 12.
Published in final edited form as: J Immunol. 2013 Aug 19;191(6):3037–3048. doi: 10.4049/jimmunol.1301289

Figure 5. Over-expression of miR-210 results in impaired proliferation and isotype switching in vitro, and downregulates genes involved in cell division and B cell activation.

Figure 5

A. Cell cycle analysis with propidium iodide of splenic B2 cells stimulated with αIgM + CD40L + IL-4 for 48 hours. Representative FACS plots are shown in the top panel and the percentage of cells in each phase of the cell cycle is quantified in the bottom panel. The average of three mice in each group ± SEM is shown.

B. Splenic B2 cells from miR-210 TG and NTG mice (8-12 weeks old) were exposed to various stimuli as indicated for 72 hours. Cellular proliferation was assessed by CFSE dilution, and the average number of divisions undergone by responding cells (division index) calculated. Top panel illustrates the gating strategy used to assess the percentage of IgG1 class-switched B-cells that have undergone 6 divisions. Histograms (bottom panel) are gated on this subset and the percentage of IgG1 class-switched B-cells quantified in the bottom right panel. Student’s t-test was performed for each condition, with n=3 in each group. * indicates p< 0.05 and ** indicates p< 0.01.