Figure 1. OVA-specific CD8⁺ T-cell frequency in mice vaccinated with polyanhydride microparticles prepared from 50:50 ratio of CPTEG:CPH. Mice were vaccinated twice at 7 d intervals with 100 µg of ovalbumin (OVA) and 50 µg of CpG ODN. Peripheral blood lymphocytes were co-stained using a fluorescently tagged tetramer (binding to OVA/MHC-specific T-cell receptor) and fluorescently labeled anti-CD3 and CD8 antibodies. All groups were statistically compared using ANOVA followed by tukey post-test (*p < 0.05). Adapted from Joshi et al. (2013).⁵⁸