Table 2. Characterization of desmosomes in resting and sNTSR3 treated HT29 cells.
| Condition | Numbera Desmosomes/Slice |
Intermediate filament associationsb | |||
|---|---|---|---|---|---|
| Desmosome score | 0 | 1 | 2 | 3 | |
| - sNTSR3 | 5±0.34 | 2% | 6% | 31% | 61% |
| + sNTSR3 | 3.7±0.31* | 31% | 25% | 23% | 15% |
a
Average number of desmosomes per 70 nm cell slice, about 160-190 desmosomes were counted from 60 electronic views for each condition.
b
Each desmosome was assigned to a score from 0-3, where 0 corresponds to no intermediate filaments visible near the desmosome, 1 corresponds to ambigous density near the intracellular plaque, 2 corresponds to a small number of intermediate filaments bundles, and 3 corresponds to strong intermediate filament bundles associated with the plaque. 189 desmosomes have been evaluated in control condition and 156 desmosomes have been evaluated in treated cells from 4 independent experiments.
*
p<0.01 (Student's t-test)