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. 2014 Jul;5(7-8):273–284. doi: 10.18632/genesandcancer.25

Figure 1. TMPRSS2-ERG splice variants and subcellular localization of the respective proteins.

Figure 1

(A) Schematic representation of TM-ERG3, TM-ERG8 and wild type ERG3 proteins. NTD, N- terminal domain; PNT, Pointed domain; CAE, Central alternative exons; CD, Central domain; ETS, DNA binding domain; CTD, C-terminal domain; U, Unique 70 amino acids (B) Expression of TM-ERG3 and TM-ERG8 proteins in HEK293 cells. Cell lysate, separated on a SDS-PAGE gel and transferred to PVDF membrane, was probed with ERG specific 9FY monoclonal antibody. TM-ERG3 and TM-ERG3-GFP correspond to 53 and 79 kDa proteins, respectively. TM-ERG8 and TM-ERG8-RFP correspond to 38 and 64 kDa proteins, respectively. Glyceraldehyde 3- phosphate dehydrogenase, GAPDH, was used as an internal control. (C) Subcellular localization of TM-ERG3-GFP and TM-ERG-RFP proteins in HEK293 cells. ERG3 was localized to the nucleus while ERG8 was found predominantly in the cytoplasm. DAPI was used for staining the nuclei.