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. 2014 Jul;5(7-8):273–284. doi: 10.18632/genesandcancer.25

Figure 2. Transcriptional activation of downstream targets by TMPRSS2-ERG splice variants.

Figure 2

(A) Modulation of expression of a luciferase reporter construct containing mef2c enhancer. The reporter construct was co-transfected with 0, 20, 40 and 80 ng of vectors expressing wtERG3, TM-ERG3, TM-ERG8 or TM-ERG3 mutant. Each assay was repeated a minimum of 3 times. The data from a representative experiment is presented here. The extent of activation is presented in the form of relative luciferase activity, which increased with increasing concentrations of vector for both wtERG3 and TM-ERG3. TM-ERG8 and TM-ERG3mut resulted in baseline levels of activity. (B) Transdominant effect of TM-ERG8 on transcriptional activation mediated by TM- ERG3. A fixed amount of luciferase reporter (40 ng) and TM-ERG3 (40 ng) were co-transfected along with an increasing amount of TM-ERG8 (0-40 ng) into HEK293 cells. A decrease in relative luciferase activity was observed with increased TM-ERG8.