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. 2014 Jul;5(7-8):273–284. doi: 10.18632/genesandcancer.25

Figure 3. Effect of ectopic expression of TMPRSS2-ERG8 in prostate cancer derived VCaP cells.

Figure 3

(A) Ectopic expression of TM-ERG8 affects the level of endogenous TM-ERG3 and C- MYC proteins in VCaP cells. Cells were transfected with different amounts of TM-ERG8 as indicated, lysed, and analyzed for protein expression of TM-ERG3 and C-MYC. Signals detected were quantitated using Odyssey software (data not shown). A decrease in endogenous TM- ERG3 protein and C-MYC protein was evident with the expression of TM-ERG8. The experiment was repeated three times. (B) Analysis of the effect of TM-ERG8 on endogenous TM-ERG3 and C-MYC at the RNA level. Quantitative RT-PCR was performed using RNA from VCaP cells transfected with TM-ERG8. Both TM-ERG3 and C-MYC remained at baseline levels of expression.