Skip to main content
. 2014 Jul 28;289(37):25393–25403. doi: 10.1074/jbc.M114.575894

FIGURE 7.

FIGURE 7.

Representation of mutations made to Rab11b, mutant ITC measurements, and subcellular localizations of PKG II and Rab11b in HeLa cells. A, schematic representation of the PKGII LZ-Rab11b complex with enlarged panels showing the interface seen in the crystal structure (left) and in a modeled structure with PKG II T62E/A66Q mutations (right). B and C, ITC was performed as described under “Experimental Procedures.” No exothermal response was seen when titrating Rab11b into PKG II LZ with a T62E mutation (B) or A66Q mutation (C). D, HeLa cells expressing PKG II-FLAG (panels 1–3) or PKG II T62E/A66Q-FLAG (panels 4–6) together with HA-Rab11b were analyzed by confocal microscopy, as described under “Experimental Procedures.” PKG II WT-FLAG (panel 1, green) and HA-Rab11b (panel 2, red) co-localized in the recycling compartment (panel 3, areas of colocalization are yellow), whereas PKG II T62E/A66Q-FLAG did not co-localize with HA-Rab11b (panels 4–6).