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Coupling of NADH consumption to substrate turnover in KshA1 and KshA5
| Enzyme | Substrate | Conc | time | Ratio |
|---|---|---|---|---|
| μm | min | Substrate/NADH | ||
| KshA1 | ADD | 40 | 5 | 1.02 (0.07)a |
| 80 | 3 | 1.2 (0.2) | ||
| 4-BNC | 20 | 3 | 1.1 (0.1) | |
| 50 | 7 | 0.95 (0.05) | ||
| KshA5 | ADD | 20 | 3 | 1.1 (0.1) |
| 40 | 5 | 0.99 (0.01) | ||
| 4-Estren-3,17-dione | 50 | 2 | 1.0 (0.1) |
a Values in parentheses represent means ± S.E.
