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. 2014 Jul 28;289(37):25546–25555. doi: 10.1074/jbc.M114.565291

FIGURE 1.

FIGURE 1.

USP17 up-regulates RORγt-mediated Il17a promoter transcriptional activity. A, the effects of DUB family members with RORγt on Il17a promoter activity. The Il17a luciferase reporter and a control β-gal luciferase reporter were cotransfected into 293T cells with the indicated 18 plasmids. Forty-eight hours later, cells were harvested, and luciferase activity was detected. B, FLAG-tagged RORγt and increasing amounts of USP17 (1 and 2 μg) or its mutant, USP17C89S, were cotransfected into 293T cells with the Il17a luciferase reporter. Forty-eight hours after transfection, cells were lysed, and luciferase activity was measured. NS, not significant. C, Myc-USP17 (3 and 6 μg) and the Il17a luciferase reporter were transfected into FLAG-RORγt-Jurkat cells by electroporation. Forty-eight hours after transfection, cells were stimulated by 12-myristate 13-acetate (PMA) and ionomycin for 12 h and then lysed for the luciferase assay. DMSO, dimethyl sulfoxide. Data are representative of more than three independent experiments, and error bars show mean ± S.D.