FIGURE 7.

The inhibitory function of tomosyn is dominant over the stimulatory activity of Munc18c. A, diagram illustrating the experimental procedures for the reconstituted fusion reactions. B, initial lipid mixing rates of the indicated SNARE-mediated fusion reactions showing the activities of tomosyn and tomosyn fragments in the presence or absence of Munc18c. Each fusion reaction contained 5 μm t-SNAREs and 1.5 μm v-SNARE. The final concentration of each SNARE regulator was at 5 μm. Data are presented as percentage of fluorescence change per 10 min. Error bars indicate S.D.