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. 2014 Jul 15;289(37):25581–25589. doi: 10.1074/jbc.M114.576108

FIGURE 4.

FIGURE 4.

Vacuolar malate relieves cytosolic ATP inhibition. A, currents elicited by a voltage ramp from +60 to −160 mV in 1.5 s (holding potential +60 mV) in the presence of a cytosolic-side buffer containing 100 mm malate and vacuolar-side buffers containing 0 mm malate (100 mm malatecyt/100 mm MESvac), 1 mm malate (100 mm malatecyt, 1 mm malate + 99 mm MESvac), 10 mm malate (100 mm malatecyt, 10 mm malate + 90 mm MESvac), 100 mm malate (100 mm malatecyt, 100 mm malatevac), in the absence or presence of 1 mm ATPfree (+ATP) in cytosolic-side buffer. B, ratios (IATP/ICtrl, mean ± S.E., n = 5–7) between currents recorded in the presence (IATP) and absence (Ictrl) of 1 mm ATPfree with the progressive increase of vacuolar malate concentration as indicated (cytosolic buffer was kept constant with 100 mm malate). The solid lines represent data fit with Equation 3 (“Experimental Procedures”; Table 1). Error bars denote S.E.