TABLE 1.
Parameters of the Boltzmann fits (Equation 3) in different experimental conditions
IATP/I0 is the ratio between the current measured in the presence of ATP and in cytosolic solutions without ATP. Iunh is the residual current in presence of ATP, V½ is the potential at which half of the current was blocked. Data are presented as mean ± S.D.
| Ionic conditions |
IATP/I0 at −160 mV | Parameters |
Location | |||
|---|---|---|---|---|---|---|
| Cytosolic | Vacuolar | ATPcyt | V½ | Iunh | ||
| mm | % | mV | % | |||
| 30 mm Malate | 100 mm Malate | 0.1 | 46 ± 1 | −131 ± 4 | 40 ± 7 | Fig. 1B |
| 30 mm Malate | 100 mm Malate | 0.5 | 17 ± 1 | −118 ± 1 | 16 ± 3 | Fig. 1B |
| 30 mm Malate | 100 mm Malate | 1 | 10 ± 1 | −114 ± 0.8 | 6 ± 2 | Fig. 1B |
| 50 mm Malate | 100 mm Malate | 1 | 19 ± 2 | −119 ± 4 | 11 ± 5 | Fig. 2B |
| 100 mm Malate | 100 mm Malate | 1 | 37 ± 2 | −135 ± 2 | 32 ± 5 | Fig. 2B |
| 100 mm Cl− | 100 mm Cl− | 1 | 29 ± 3 | −66 ± 1 | 24 ± 1 | Fig. 3B |
| 100 mm Cl− | 100 mm Malate | 1 | 34 ± 2 | −124 ± 1 | 31 ± 2 | Fig. 3B |
| 100 mm Malate | 0 mm Malate | 1 | 44 ± 2 | −112 ± 4 | 38 ± 4 | Fig. 4B |
| 100 mm Malate | 1 mm Malate | 1 | 42 ± 1 | −113 ± 2 | 39 ± 3 | Fig. 4B |
| 100 mm Malate | 10 mm Malate | 1 | 38 ± 3 | −119 ± 3 | 31 ± 4 | Fig. 4B |
| 100 mm Malate | 100 mm Cl− | 1 | 41 ± 3 | −135 ± 13 | 7 ± 16 | Fig. 4B |