FIGURE 2.

Single time traces of the SynCph2(1-2) P_r → P_fr transition measured at 665 nm. Here, the intermediates show alternately lower or higher absorbance than the P_r reference state and can be observed without further data analysis. A–D show the time traces with increasing time scales; the approximate appearance of intermediates is indicated by arrows. The intermediate lifetimes are also presented. The final intermediate R3 exhibits similar absorption as the P_fr conformation (D). The initial 10% of the time traces prior to the laser pulse are recorded to determine the baseline level; at t = 0 the laser flash triggered the photoconversion. E, individual time traces (A–D) were assembled. Here, the fit of the resulting data is shown in green as well as the residuals (below).