TABLE 4.

Kinetic parameters of FgFCO1 for substrate hydrolysis

H-D, H-disaccharide; 3-FL, 3-fucosyllactose (see Fig. 2).

Substrate	pNP-Fuc	2′-FL	H-D	3-FL	Lewisa trisaccharide	Lewisx trisaccharide
kcat/Km (m−1s−1)a	12	625	0.55	ND	NDb	–c

^a Steady-state kinetics were performed at 37 °C in 50 mm sodium acetate, at pH 5. The standard deviation of k_cat/K_m was less than 20%.

^b ND, no detectable activity.

^c Not stable in solution.