FIGURE 8.

AXL soluble receptors inhibit MERTK-driven efferocytosis. A, MCF10A-MERTK and MCF10A-pMSCV labeled with PKH26-red were co-cultured with PKH67-green apoptotic CEM for 3 h in the presence or absence of 1 μm human sAXL and efferocytosis was analyzed by flow cytometry. B, a similar experiment was performed as described in A with MDA-MB 231 instead of MCF10A phagocytes. C, efferocytosis was carried as in panel A for 4 h and analyzed by Amnis Imagestream. However, instead of apoptotic CEM cells, apoptotic MCF10A cells were used in this experiment.