FIGURE 10.

A, immunoblot analyses of the chromatography fractions of the mouse serum collected from mice that were implanted SW620 tumor (after 12 days inoculation) with (Serum + FBP)/without (Serum) the addition of FBP (3 mm) using the antibody PabPKM2. The fractions corresponding to dimer and tetramer PKM2 are indicated. B, HUVEC adhesions to PKM2-or R399E-coated culture plates were measured by counting the attached cells after extensive washing. The cell attachments are presented as relative attachment by defining the cell attachment in the plate on which rPKM2 was coated as 100%. The p value is presented as NS (statistically insignificant; p > 0.05) and was calculated using unpaired two-tailed student's t test. The error bars are S.D. of five repeating experiments.