TABLE 1.

Characteristics of proxy secretory and endomembrane protein-encoding mRNAs and their encoded proteins

Category	Gene	Protein product	Intracellular location	mRNA properties (length)			Signal propertiesa
				5′-UTR	CDS	3′-UTR	SS	TM	HS
				nt
Cargo	B2M	β2-Microglobulin	Secreted	60	360	567	Yes	No	1.581
	VEGFA	Vascular endothelial growth factor A	Secreted	498	1239	1940	Yes	No	0.585
	THPO	Thrombopoietin	Secreted	215	1062	528	Yes	No	1.5
Endo	GRP94/HSP90B1	Heat shock protein 90 kDa β	ER lumen	105	2412	263	Yes	No	1.327
	BiP/HSPA5	Heat shock 70-kDa protein 5	ER lumen	261	1965	1747	Yes	No	1.384
	CALR	Calreticulin	ER lumen	80	1254	595	Yes	No	2.089

^a Signal properties are as follows. SS, signal sequence; TM, transmembrane domain; HS, hydrophobicity score (average hydrophobicity score of the signal peptide calculated using Kyte-Doolittle amino acid hydrophobicity scores).