TABLE 5.

Lipopolysaccharide-binding parameters and amidase activities of the N-terminal substitution mutants of factor C

	ka	kd	Ka	Activation by lipopolysaccharides
	m−1s−1	s−1	m−1
Wild-type	4.29 × 106 ± 2.50 × 106	3.25 × 10−3 ± 0.42 × 10−3	1.32 × 109	Yes
R1H-factor C	8.26 × 106 ± 2.76 × 106	1.41 × 10−2 ± 0.92 × 10−2	0.59 × 109	No
R1A-factor C	6.41 × 106 ± 3.87 × 106	1.74 × 10−2 ± 0.87 × 10−2	0.37 × 109	No
R1L-factor C	5.80 × 106 ± 4.15 × 106	5.79 × 10−3 ± 0.30 × 10−3	1.00 × 109	No
R1K-factor C	6.85 × 106 ± 3.73 × 106	6.30 × 10−3 ± 1.28 × 10−3	1.09 × 109	Yes